The discovery of Halictivirus resolves the Sinaivirus phylogeny.
Identifieur interne : 000221 ( Main/Exploration ); précédent : 000220; suivant : 000222The discovery of Halictivirus resolves the Sinaivirus phylogeny.
Auteurs : Diane Bigot [France] ; Anne Dalmon [France] ; Bronwen Roy [Australie] ; Chunsheng Hou [République populaire de Chine] ; Michèle Germain [France] ; Manon Romary [France] ; Shuai Deng [République populaire de Chine] ; Qingyun Diao [République populaire de Chine] ; Lucy A. Weinert [France] ; James M. Cook [Australie] ; Elisabeth A. Herniou [France] ; Philippe Gayral [France]Source :
- The Journal of general virology [ 1465-2099 ] ; 2017.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Protéines virales, Virus des insectes.
- isolement et purification : Virus des insectes.
- virologie : Abeilles, Fourmis.
- Analyse de séquence d'ADN, Animaux, Phylogénie, Variation génétique, Virus des insectes.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Viral Proteins.
- classification : Insect Viruses.
- genetics : Insect Viruses.
- isolation & purification : Insect Viruses.
- virology : Ants, Bees.
- Animals, Genetic Variation, Phylogeny, Sequence Analysis, DNA.
Abstract
By providing pollination services, bees are among the most important insects, both in ecological and economical terms. Combined next-generation and classical sequencing approaches were applied to discover and study new insect viruses potentially harmful to bees. A bioinformatics virus discovery pipeline was used on individual Illumina transcriptomes of 13 wild bees from three species from the genus Halictus and 30 ants from six species of the genera Messor and Aphaenogaster. This allowed the discovery and description of three sequences of a new virus termed Halictus scabiosae Adlikon virus (HsAV). Phylogenetic analyses of ORF1, RNA-dependent RNA-polymerase (RdRp) and capsid genes showed that HsAV is closely related to (+)ssRNA viruses of the unassigned Sinaivirus genus but distant enough to belong to a different new genus we called Halictivirus. In addition, our study of ant transcriptomes revealed the first four sinaivirus sequences from ants (Messor barbarus, M. capitatus and M. concolor). Maximum likelihood phylogenetic analyses were performed on a 594 nt fragment of the ORF1/RdRp region from 84 sinaivirus sequences, including 31 new Lake Sinai viruses (LSVs) from honey bees collected in five countries across the globe and the four ant viral sequences. The phylogeny revealed four main clades potentially representing different viral species infecting honey bees. Moreover, the ant viruses belonged to the LSV4 clade, suggesting a possible cross-species transmission between bees and ants. Lastly, wide honey bee screening showed that all four LSV clades have worldwide distributions with no obvious geographical segregation.
DOI: 10.1099/jgv.0.000957
PubMed: 29058655
Affiliations:
- Australie, France, République populaire de Chine
- Centre-Val de Loire, Languedoc-Roussillon, Occitanie (région administrative), Région Centre
- Montpellier, Pékin, Tours
- Université François-Rabelais de Tours
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Le document en format XML
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uniqKey="Dalmon A" first="Anne" last="Dalmon">Anne Dalmon</name><affiliation wicri:level="1"><nlm:affiliation>2INRA UR 406 Abeilles et environnement, Centre de recherche Provence-Alpes-Côte d'Azur, Site Agroparc, Domaine St Paul 228, Route de l'aérodrome CS40509 84914 Avignon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>2INRA UR 406 Abeilles et environnement, Centre de recherche Provence-Alpes-Côte d'Azur, Site Agroparc, Domaine St Paul 228, Route de l'aérodrome CS40509 84914 Avignon</wicri:regionArea><wicri:noRegion>40509 84914 Avignon</wicri:noRegion><wicri:noRegion>40509 84914 Avignon</wicri:noRegion></affiliation></author><author><name sortKey="Roy, Bronwen" sort="Roy, Bronwen" uniqKey="Roy B" first="Bronwen" last="Roy">Bronwen Roy</name><affiliation wicri:level="1"><nlm:affiliation>3Hawkesbury Institute for the Environment, Western Sydney University, Locked Bag 1797, Penrith NSW 2751, Australia.</nlm:affiliation><country 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Recherche sur la Biologie de l'Insecte, UMR 7261, CNRS, Université de Tours, 37200 Tours</wicri:regionArea><placeName><region type="region" nuts="2">Centre-Val de Loire</region><region type="old region" nuts="2">Région Centre</region><settlement type="city">Tours</settlement><settlement type="city">Tours</settlement></placeName><orgName type="university">Université François-Rabelais de Tours</orgName></affiliation></author><author><name sortKey="Dalmon, Anne" sort="Dalmon, Anne" uniqKey="Dalmon A" first="Anne" last="Dalmon">Anne Dalmon</name><affiliation wicri:level="1"><nlm:affiliation>2INRA UR 406 Abeilles et environnement, Centre de recherche Provence-Alpes-Côte d'Azur, Site Agroparc, Domaine St Paul 228, Route de l'aérodrome CS40509 84914 Avignon, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>2INRA UR 406 Abeilles et environnement, Centre de recherche Provence-Alpes-Côte d'Azur, Site Agroparc, Domaine St Paul 228, Route de l'aérodrome CS40509 84914 Avignon</wicri:regionArea><wicri:noRegion>40509 84914 Avignon</wicri:noRegion><wicri:noRegion>40509 84914 Avignon</wicri:noRegion></affiliation></author><author><name sortKey="Roy, Bronwen" sort="Roy, Bronwen" uniqKey="Roy B" first="Bronwen" last="Roy">Bronwen Roy</name><affiliation wicri:level="1"><nlm:affiliation>3Hawkesbury Institute for the Environment, Western Sydney University, Locked Bag 1797, Penrith NSW 2751, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>3Hawkesbury Institute for the Environment, Western Sydney University, Locked Bag 1797, Penrith NSW 2751</wicri:regionArea><wicri:noRegion>Penrith NSW 2751</wicri:noRegion></affiliation></author><author><name sortKey="Hou, Chunsheng" sort="Hou, Chunsheng" uniqKey="Hou C" first="Chunsheng" last="Hou">Chunsheng Hou</name><affiliation wicri:level="1"><nlm:affiliation>4Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>4Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Germain, Michele" sort="Germain, Michele" uniqKey="Germain M" first="Michèle" last="Germain">Michèle Germain</name><affiliation wicri:level="4"><nlm:affiliation>1Institut de Recherche sur la Biologie de l'Insecte, UMR 7261, CNRS, Université de Tours, 37200 Tours, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>1Institut de Recherche sur la Biologie de l'Insecte, UMR 7261, CNRS, Université de Tours, 37200 Tours</wicri:regionArea><wicri:noRegion>37200 Tours</wicri:noRegion><orgName type="university">Université François-Rabelais de Tours</orgName><placeName><settlement type="city">Tours</settlement><region type="old region" nuts="2">Région Centre</region><region type="region" nuts="2">Centre-Val de Loire</region></placeName></affiliation></author><author><name sortKey="Romary, Manon" sort="Romary, Manon" uniqKey="Romary M" first="Manon" last="Romary">Manon Romary</name><affiliation wicri:level="4"><nlm:affiliation>1Institut de Recherche sur la Biologie de l'Insecte, UMR 7261, CNRS, Université de Tours, 37200 Tours, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>1Institut de Recherche sur la Biologie de l'Insecte, UMR 7261, CNRS, Université de Tours, 37200 Tours</wicri:regionArea><wicri:noRegion>37200 Tours</wicri:noRegion><orgName type="university">Université François-Rabelais de Tours</orgName><placeName><settlement type="city">Tours</settlement><region type="old region" nuts="2">Région Centre</region><region type="region" nuts="2">Centre-Val de Loire</region></placeName></affiliation></author><author><name sortKey="Deng, Shuai" sort="Deng, Shuai" uniqKey="Deng S" first="Shuai" last="Deng">Shuai Deng</name><affiliation wicri:level="1"><nlm:affiliation>4Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>4Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Diao, Qingyun" sort="Diao, Qingyun" uniqKey="Diao Q" first="Qingyun" last="Diao">Qingyun Diao</name><affiliation wicri:level="1"><nlm:affiliation>4Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>4Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Weinert, Lucy A" sort="Weinert, Lucy A" uniqKey="Weinert L" first="Lucy A" last="Weinert">Lucy A. Weinert</name><affiliation wicri:level="3"><nlm:affiliation>6Institut des Sciences de l'Evolution UMR5554, Université Montpellier-CNRS-IRD-EPHE, Montpellier, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>6Institut des Sciences de l'Evolution UMR5554, Université Montpellier-CNRS-IRD-EPHE, Montpellier</wicri:regionArea><placeName><region type="region">Occitanie (région administrative)</region><region type="old region">Languedoc-Roussillon</region><settlement type="city">Montpellier</settlement></placeName></affiliation></author><author><name sortKey="Cook, James M" sort="Cook, James M" uniqKey="Cook J" first="James M" last="Cook">James M. Cook</name><affiliation wicri:level="1"><nlm:affiliation>3Hawkesbury Institute for the Environment, Western Sydney University, Locked Bag 1797, Penrith NSW 2751, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>3Hawkesbury Institute for the Environment, Western Sydney University, Locked Bag 1797, Penrith NSW 2751</wicri:regionArea><wicri:noRegion>Penrith NSW 2751</wicri:noRegion></affiliation></author><author><name sortKey="Herniou, Elisabeth A" sort="Herniou, Elisabeth A" uniqKey="Herniou E" first="Elisabeth A" last="Herniou">Elisabeth A. Herniou</name><affiliation wicri:level="4"><nlm:affiliation>1Institut de Recherche sur la Biologie de l'Insecte, UMR 7261, CNRS, Université de Tours, 37200 Tours, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>1Institut de Recherche sur la Biologie de l'Insecte, UMR 7261, CNRS, Université de Tours, 37200 Tours</wicri:regionArea><wicri:noRegion>37200 Tours</wicri:noRegion><orgName type="university">Université François-Rabelais de Tours</orgName><placeName><settlement type="city">Tours</settlement><region type="old region" nuts="2">Région Centre</region><region type="region" nuts="2">Centre-Val de Loire</region></placeName></affiliation></author><author><name sortKey="Gayral, Philippe" sort="Gayral, Philippe" uniqKey="Gayral P" first="Philippe" last="Gayral">Philippe Gayral</name><affiliation wicri:level="4"><nlm:affiliation>1Institut de Recherche sur la Biologie de l'Insecte, UMR 7261, CNRS, Université de Tours, 37200 Tours, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>1Institut de Recherche sur la Biologie de l'Insecte, UMR 7261, CNRS, Université de Tours, 37200 Tours</wicri:regionArea><wicri:noRegion>37200 Tours</wicri:noRegion><orgName type="university">Université François-Rabelais de Tours</orgName><placeName><settlement type="city">Tours</settlement><region type="old region" nuts="2">Région Centre</region><region type="region" nuts="2">Centre-Val de Loire</region></placeName></affiliation></author></analytic><series><title level="j">The Journal of general virology</title><idno type="eISSN">1465-2099</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Ants (virology)</term><term>Bees (virology)</term><term>Genetic Variation</term><term>Insect Viruses (classification)</term><term>Insect Viruses (genetics)</term><term>Insect Viruses (isolation & purification)</term><term>Phylogeny</term><term>Sequence Analysis, DNA</term><term>Viral Proteins (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Abeilles (virologie)</term><term>Analyse de séquence d'ADN</term><term>Animaux</term><term>Fourmis (virologie)</term><term>Phylogénie</term><term>Protéines virales (génétique)</term><term>Variation génétique</term><term>Virus des insectes ()</term><term>Virus des insectes (génétique)</term><term>Virus des insectes (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Insect Viruses</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Insect Viruses</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines virales</term><term>Virus des insectes</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Insect Viruses</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus des insectes</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Abeilles</term><term>Fourmis</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Ants</term><term>Bees</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Genetic Variation</term><term>Phylogeny</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Animaux</term><term>Phylogénie</term><term>Variation génétique</term><term>Virus des insectes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">By providing pollination services, bees are among the most important insects, both in ecological and economical terms. Combined next-generation and classical sequencing approaches were applied to discover and study new insect viruses potentially harmful to bees. A bioinformatics virus discovery pipeline was used on individual Illumina transcriptomes of 13 wild bees from three species from the genus Halictus and 30 ants from six species of the genera Messor and Aphaenogaster. This allowed the discovery and description of three sequences of a new virus termed Halictus scabiosae Adlikon virus (HsAV). Phylogenetic analyses of ORF1, RNA-dependent RNA-polymerase (RdRp) and capsid genes showed that HsAV is closely related to (+)ssRNA viruses of the unassigned Sinaivirus genus but distant enough to belong to a different new genus we called Halictivirus. In addition, our study of ant transcriptomes revealed the first four sinaivirus sequences from ants (Messor barbarus, M. capitatus and M. concolor). Maximum likelihood phylogenetic analyses were performed on a 594 nt fragment of the ORF1/RdRp region from 84 sinaivirus sequences, including 31 new Lake Sinai viruses (LSVs) from honey bees collected in five countries across the globe and the four ant viral sequences. The phylogeny revealed four main clades potentially representing different viral species infecting honey bees. Moreover, the ant viruses belonged to the LSV4 clade, suggesting a possible cross-species transmission between bees and ants. Lastly, wide honey bee screening showed that all four LSV clades have worldwide distributions with no obvious geographical segregation.</div></front></TEI><affiliations><list><country><li>Australie</li><li>France</li><li>République populaire de Chine</li></country><region><li>Centre-Val de Loire</li><li>Languedoc-Roussillon</li><li>Occitanie (région administrative)</li><li>Région Centre</li></region><settlement><li>Montpellier</li><li>Pékin</li><li>Tours</li></settlement><orgName><li>Université François-Rabelais de Tours</li></orgName></list><tree><country name="France"><region name="Centre-Val de Loire"><name sortKey="Bigot, Diane" sort="Bigot, Diane" uniqKey="Bigot D" first="Diane" last="Bigot">Diane Bigot</name></region><name sortKey="Dalmon, Anne" sort="Dalmon, Anne" uniqKey="Dalmon A" first="Anne" last="Dalmon">Anne Dalmon</name><name sortKey="Gayral, Philippe" sort="Gayral, Philippe" uniqKey="Gayral P" first="Philippe" last="Gayral">Philippe Gayral</name><name sortKey="Germain, Michele" sort="Germain, Michele" uniqKey="Germain M" first="Michèle" last="Germain">Michèle Germain</name><name sortKey="Herniou, Elisabeth A" sort="Herniou, Elisabeth A" uniqKey="Herniou E" first="Elisabeth A" last="Herniou">Elisabeth A. Herniou</name><name sortKey="Romary, Manon" sort="Romary, Manon" uniqKey="Romary M" first="Manon" last="Romary">Manon Romary</name><name sortKey="Weinert, Lucy A" sort="Weinert, Lucy A" uniqKey="Weinert L" first="Lucy A" last="Weinert">Lucy A. Weinert</name></country><country name="Australie"><noRegion><name sortKey="Roy, Bronwen" sort="Roy, Bronwen" uniqKey="Roy B" first="Bronwen" last="Roy">Bronwen Roy</name></noRegion><name sortKey="Cook, James M" sort="Cook, James M" uniqKey="Cook J" first="James M" last="Cook">James M. Cook</name></country><country name="République populaire de Chine"><noRegion><name sortKey="Hou, Chunsheng" sort="Hou, Chunsheng" uniqKey="Hou C" first="Chunsheng" last="Hou">Chunsheng Hou</name></noRegion><name sortKey="Deng, Shuai" sort="Deng, Shuai" uniqKey="Deng S" first="Shuai" last="Deng">Shuai Deng</name><name sortKey="Diao, Qingyun" sort="Diao, Qingyun" uniqKey="Diao Q" first="Qingyun" last="Diao">Qingyun Diao</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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